Hi Mark,
very good questions!
first I just wrote the obvious answers:
for all the tests I’ve shown, and for my typical work,
I always and constantly have a single Pro16 connected.
absolutely nothing special…
BUT… I then wanted to explain my vague phrasing (for the tests / typically / nothing special)
… because at the same time recently I also wrestle with a completely different topic
that USB3 is not working anymore at all (ticket 67883).
… and just to be totally sure I’ve picked up another “good” Pro16 which still works fine with USB3
and – DRUM ROLL – the “cannot save/reload last preset” is gone!!!
so a bit more testing shows to me: at least when there is this connect problem

the last/saved default prefix is not used anymore, Logic2 starts up with that (builtin?)
2digital and 2analog 1.2V setting, no matter what was saved before!
this USB2-only error stops the normal startup and breaks default prefix,
even if that setup was “tuned” to work (and was working before) in USB2 mode with the same device
one more resaon to get our broken Pro16 USB connector fixed…
so thanks a lot for asking the “typical” support questions again;)
Harald